User manuals, guides and specifications for your ge akta start laboratory equipment. Akta pure 25 purification system is intended for microgramscale protein purifications in tricorn columns up to milligramscale using hitrap and hiscreen columns. Troubleshooting check that the conductivity signal is stable before sample injection check the gradient performance possible to identify when the buffer has been completely exchangedwashed out. Priming system and sample pumps for aktapure and aktaavant. Fraction collector frac950 user manual 181956 edition ae 7 1 introduction 1. Unicorn 7 features an intuitive interface for creating methods by dragging and dropping predefined phases for a wide range of techniques. Introduction to column resins and chromatography separation methods 4.
Gloves and protective eyewear should be worn when handling samples and reagents buffers, however it is preferable that the user remove gloves prior to entering commands via the computer keyboard or mouse. A full description of how to operate unicorn is found in the unicorn user manual. Scope of this manual the operating instructions cover akta pure 25 and akta pure 150 instruments, using previously created methods in unicorn software. Introduction to the akta pure systems hardware and software 3. Cytiva formerly ge healthcare life sciences 16,785 views. Manual load position of the injection valve that allows the user to load the sample loop with sample by depressing the plunger of inserted syringe full. Part function 1 multimodulepanel 2 inletvalve 3 pumprinsingliquidtube 4 systempumpb 5 pressuremonitor 6 systempumpa 7 mixer 8 outletvalve 9 injectionvalve. Weitere details finden sie in akta pure user manual. Akta explorer systems include an extensive column library with details of run parameters for each prepacked column. Aktaexplorer am ers ha m bio scie nce am er sham bio ci n a m e r s h a m b io s c i en c a m e r s h a m b io s c i e n c e akta system training guide explorer 100 10.
Akta pure chromatography system is a highly versatile, modular system with a number of design features to facilitate reliable purification. Akta ready system 1 inlet manifold, 2 pump tubing, and 3 main flow path. It will help if they get w10 with some good dev support when it comes out. Akta pure user manual ge healthcare life sciences manualzz. In the unicorn software, prompt the system control window. Akta ready is a chromatography system using a disposable flow path. The system consists of the akta pure instrument and. Database contains 4 ge akta start manuals available for free online viewing or downloading in pdf. Falls unicorn keine verbindung mit dem ausgewahlten gerat herstellen kann, siehe fehlersuche in akta pure user manual. The small diameter, low flow kit manages flows of up to 175 lh using columns typically less than 20 cm. Gloves and protective eyewear should be worn when handling samples and reagents buffers, however it is preferable that the user remove gloves prior to entering commands via. Easily share your publications and get them in front of issuus. Length mounted mm label description tubing b1andb2 mounted b1b7 inletstoinletvalveb fep, o. A a basic system configuration for convenient protein purification.
Akta pure cytiva, formerly ge healthcare life sciences. Aug 24, 2018 if you are tied to your akta protein purification system during manual runs, learn how to get started with quick method creation using unicorn control software. Protein purification laboratory of molecular biology. It also contains an overview of the safety instructions. This high flow rate is necessary when working with. Sample loops can be filled manually, via a syringe, or with a sample pump.
Operate in accordance with local safety instructions. Aktacrossflow is intended for laboratory use only, not for clinical or in vitro use, or for diagnostic purposes. Data file 28915986 ad readytoprocess akta ready system fig 1. Tubing kit changes aktapurifier 100 high flow at delivery 0. Ge akta explorer certified fplc system gmi trusted lab. Apr 01, 2015 issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. All users must read the safety instructions in the aktapilot operating instructions to fully understand the safe use of the aktapilot system, before installing, using, or maintaining the system. Introduction to this handbook and unicorn aktadesign purification user manual 8 2423 edition ad about this handbook 5. Check valve is clogged or damaged, causing leakage. Aktaexplorer am ers ha m bio scie nce am er sham bio ci n a m e r s h a m b io s c ie n c a m e r s h a m b io s c ie box n c e monitor conductivity ph uv detector 3 wavelengths pumps column valves injector sample valve buffers phcell electrode sample holder pump a buffer switching valve outlet valve. Ge pharmacia akta purifier 100 gmi trusted laboratory. I tried purging the pump multiple times but no success. Akta pure, protein purification your way april scharfstein. Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online.
The product must also be used in the same state as it was delivered from ge healthcare. Two examples of system configurations for akta pure 25 showing positions of modules on the front panel and flow paths for each. It allows users to speed up process scaleup and cleaning between product. Exterior design akta pure has a modular design, with all liquid handling modules placed on the exterior of the instrument. Fully scalable, the unicorn platform is suitable for use within smallscale research all the way to fullscale manufacturing. Fplc standard operating procedure akta purifier system page page 2 of 9 check there is sufficient volumes of buffers, mq and etoh 20% for the coming runs. Akta pure 25, designed for a broad range of applications and purification tasks in a multiuser environment, and.
Function the fraction collector collects fractions from akta pure purification runs. Akta pure 25 new owners intro the exercise below will give a quick demonstration of how easy and intuitive the akta pure 25 will be for you in demonstrating downstream processing to your students. Akta pure is a flexible and intuitive purification system for proteins, peptides, and nucleic acids from microgram levels to tens of grams of target product in research applications. Unicorn 7 cytiva, formerly ge healthcare life sciences.
Always keep the he inlet above the solution or close the valve. For fast protein liquid chromatography of tens of grams of protein, akta pure 150 can be used with flow rates up to 150 mlmin. Ensure your akta system is operating at peakperformance by. It is intended to be operated as an integrated part of an aktadesign chromatography system running unicorn software. The akta purifier 100 is perfect for application spanning a wide range of wavelengths the akta purifier 100 allows for rate flows of up to 100 ml per minute fast and easy start to purification work preprogrammed application protocols and method templates speed and reliability for every run fast optimisation and peak identificationaccurate result comparisons and peakfractionation column. Akta fplc system operators manual marshall scientific by. When the user selects a column, the akta explorer instinctively sets the column volume, flow rate and pressure limit parameters into the method template.
Fraction collector frac950 boyce thompson institute. For further details see akta avant user manual and akta pure user manual. How can i get rid of air in an akta purifier inlet tube. Twostep purification with akta pure, or akta avant, using loop.
Air ventilation uv monitor when using 23 wavelengths pump movement mixer changing valve position fraction collector movements for long term storage. If the leakage persists, replace the assembly according to instructions in the pump p960 user manual. Twostep purification with akta pure, or akta avant, using loop collection cue card contents principles 2 setup for multistep purification 2 unicorn methods 3 how to add user defined phases to a method 3 description of the user defined phase in method one 4 edit sample application phase in method two 5 recommendations 6 introduction this cue card describes how to configure akta pure. Aktaprime plus user manual 11002644 edition aa 9 1introduction this section is an introduction to aktaprime plus, the documentation included and the content in this manual. Technical details about the instrument and the individual modules are found in akta pure user manual. The system is designed for process development, process scaleup and scale. Once the lines have been washed with water, you will need to start a manual run before attaching your column to the system.
Unicorn efficient method creation is often about finding useful tips on how to develop methods easy to maintain, reuse, and share. The system consists of the akta pcc instrument and unicorn system control software. Akta pure is a flexible chromatography system for the reliable purification of proteins, peptides, and nucleic acids at laboratory scale. Akta fplc system operators manual marshall scientific. Check that injection valve is set to load column position. I recently ran many samples on our akta pure without any intermittent cleaning. The aim of this tutorial is to demonstrate the use of the ubiquitous akta systems and prepacked hitrap chromatography media for rapid, mediumscale protein purification.
Opening the unicorn software windows for manual control and programmable features purgingpriming pumps and tubing. Do not use in a dusty atmosphere or close to spraying water. Once the lines have been washed with water, you will need to start a. All users must read this entire manual to fully understand the safe use of akta. It also includes information on optional equipment. Using the akta prime plus october 22, 2012 3 j e n n i f e r a r r u d a attaching the column and washing the ethanol from the column. Systems provide more control than manual purification because of the ability to automatically control the flow rate and monitor the progress of the purification as well as to make controlled gradients and automatically collect fractions.
Fraction collector frac950 user manual 181956 edition ae 9 1. This video show how to set up a two step method in the unicorn software for the akta pure protein. File containing module user manuals and component instructions for aktafplc equipment pump p920 user manual monitor upc900 user manual. Get familiarized with its use and have its instruction manual handy. Unicorn system control software provides builtin knowledge for planning and controlling runs, as well as analyzing results. A simple, yet flexible, control software for akta protein purification systems. Ge akta pure operating instructions manual pdf download. For further details see akta avant user manual and akta pure user manual references on page 14. Jan 09, 2018 akta pure, protein purification your way. The software can control chromatography, bioreactor, and filtration systems fig 1. Important user information all users must read this entire manual to fully understand the safe use of aktacrossflow.312 1495 580 229 1368 920 1406 557 1419 1397 1059 1210 1034 1324 773 510 853 358 1231 1506 579 726 640 51 1341 634 1194 852 714 425 1299 1318 230 583 1398 864 416 785 1259 169 1410 830 233 1465 1077 942 1227 1058